housekeeping gene β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)

Journal: Biomedical Reports

Article Title: Intronic hexanucleotide repeat expansion in TYMS in monozygotic twins with congenital progressive universal melanosis

doi: 10.3892/br.2025.2016

Figure Lengend Snippet: Expression of TYMS mRNA and protein in fibroblast cells. Mean TYMS (A) mRNA and (B) protein expression in controls (n=4) and patients (n=2). The signal intensity was normalized to β-actin. The blots were obtained from the same PVDF membrane, with TYMS and β-actin detected in separate experiments. TYMS, thymidylate synthase.

Article Snippet: The relative protein expression levels were normalized to the housekeeping gene β-actin (13E5) Rabbit mAb (1:1,000, Cat. No. 7087; Cell Signaling Technology, Inc.).

Techniques: Expressing, Membrane

Journal: Translational stroke research

Article Title: Phosphodiesterase 10A Inhibition Leads to Brain Region-Specific Recovery Based on Stroke Type.

doi: 10.1007/s12975-020-00819-8

Figure Lengend Snippet: Fig. 2 TAK-063 treatment-mediated alterations in BDNF expression in striatal tissue. BDNF expression levels measured with tissue ELISA showed elevation at 3 weeks post-striatal stroke in striatal tissue, ipsilateral to the infarct. Treatment with TAK-063 resulted in a significant increase in BDNF levels in the stroke + 3.0 mg/kg versus stroke + vehicle group in ipsilateral striatum; P = 0.005, corrected for multiple comparisons (df = 18, t value = 5.982). Error bars represent mean ± SEM for n = 10 per group

Article Snippet: To measure proBDNF, p75NTR, and pTrkB protein levels, the following kits were used: BDNF (mouse) ELISA kit (Abnova), proBDNF Rapid ELISA Kit (Biosensis), Mouse NGF R/TNFRSF16 DuoSet ELISA (R&D Systems), and PathScan® Phospho-TrkB (panTyr) Sandwich ELISA (Cell Signaling Technologies).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Molecules

Article Title: Protective Effects of Gintonin on Reactive Oxygen Species-Induced HT22 Cell Damages: Involvement of LPA1 Receptor-BDNF-AKT Signaling Pathway

doi: 10.3390/molecules26144138

Figure Lengend Snippet: Effect of gintonin on the activation of TrkB receptors in IAA-treated HT22 cells. ( A ) Representative microscopy images of TrkB phosphorylation. HT22 cells were exposed to IAA (5 μM, 2 h) and treated with gintonin (3 μg/mL, 24 h) or BDNF (30 ng/mL, 24 h). Phosphorylated TrkB is dyed green. Microscopy images were captured at the same magnification, scale bar = 100 μm. ( B ) Quantitative analysis of the images obtained from fluorescence microscopy. ( C ) The level of phosphorylation of TrkB receptors measured using a phosphor-TrkB ELISA kit. The data are expressed as the mean ± standard error of the mean (SEM; n = 4). # p < 0.05 and ## p < 0.01 compared to the control group; ** p < 0.01 compared to the IAA-treated group.

Article Snippet: The level of TrkB receptor phosphorylation in cells was measured using the PathScan ® Phospho-TrkB ELISA Kit (Cell Signaling Technology, Beverly, MA, USA), according to the manufacturer’s instructions.

Techniques: Activation Assay, Microscopy, Phospho-proteomics, Fluorescence, Enzyme-linked Immunosorbent Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Burst-Like Subcutaneous Electrical Stimulation Induces BDNF-Mediated, Cyclotraxin B-Sensitive Central Sensitization in Rat Spinal Cord

doi: 10.3389/fphar.2018.01143

Figure Lengend Snippet: Expression levels of BDNF (A) and pTrkB (B) in the L3–L5 laminae of dorsal lumbar spinal cord of rats submitted to the high-frequency SES protocol. Values are means ± SEM ( n = 6 rats per group). BDNF and pTrkB were measured with ELISA test. The BDNF was expressed as pg BDNF/mg protein due to the absorbance was compared with a concentration reference curve, while that for pTrkB we have a not a reference curve. Asterisks show a significant difference ( ∗ p < 0.05) respect to time zero (black bar) (Kruskal–Wallis test followed by the post hoc Dunn’s multiple comparisons test).

Article Snippet: BDNF protein was quantified using the ELISA E max Immunoassay System (Promega, Madison, WI, United States), while for quantifying pTrkB the ELISA test PathScan ® Phospho-TrkB (panTyr) (Cell Signaling Technology, Danvers, MA, United States) was used.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay